stamapper  (Spatial Transcriptomics Inc)

Journal: Genome Biology

Article Title: High-precision cell-type mapping and annotation of single-cell spatial transcriptomics with STAMapper

doi: 10.1186/s13059-025-03773-6

Figure Lengend Snippet: Illustration of STAMapper and its applications. a STAMapper can annotate scST data obtained from mainstream technologies such as image-based and seq-based by leveraging well-annotated sc/snRNA-seq data sequenced from microfluidics-based or droplet-based technologies. b STAMapper models genes and cells as two types of heterogeneous nodes and connects sc/scRNA-seq and scST data by their expression on the shared genes. c STAMapper takes the expression and the heterogeneous relationships of nodes as input. STAMapper then learns embeddings for cells and genes based on the information propagation mechanism on the heterogeneous graph network to fit cell labels from scRNA-seq data by using a graph attention classifier, ultimately utilizing the learned weights of information propagation on the graph to transfer cell labels on spatial data. d The output of STAMapper can be applied for annotation on large-scale scST data, reannotation on scST data, unknown cell-types detection, and gene module extraction

Article Snippet: Fig. 3 Application of STAMapper to MERFISH retina datasets. a Performance comparison of STAMapper and scANVI, RCTD, Tangram, where each box represents the method’s performance on the 50 paired datasets (five scRNA-seq datasets and ten single-cell spatial transcriptomics datasets). b UMAP plots of mouse retinal dataset (VZG105a_WT3) cells colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD using the mouse_LD_60 scRNA-seq dataset as the reference.

Techniques: Expressing, Extraction

Journal: Genome Biology

Article Title: High-precision cell-type mapping and annotation of single-cell spatial transcriptomics with STAMapper

doi: 10.1186/s13059-025-03773-6

Figure Lengend Snippet: Benchmarking cell annotation performance of STAMapper. a Overview of all datasets used for evaluating the performance of STAMapper. We collected 81 single-cell spatial transcriptomics datasets comprising a total of 344 slices, where each dataset is matched with corresponding single-cell transcriptomics data (or scRNA-seq data). b Performance comparison of STAMapper and scANVI, RCTD, Tangram regarding cell annotation accuracy on 81 pairs of scRNA-seq and single-cell spatial transcriptomics datasets. P values were calculated by paired t test. c Performance comparison of the classification accuracies of STAMapper and three other methods on different down-sampling rates (1.0, 0.8, 0.6, 0.4, 0.2) for read counts, where the down-sampling rate of 1.0 means the raw data. The upper panel depicts spatial transcriptomics datasets with more than 200 genes for sequencing (47 datasets), while the lower panel corresponds to fewer than 200 genes (34 datasets)

Article Snippet: Fig. 3 Application of STAMapper to MERFISH retina datasets. a Performance comparison of STAMapper and scANVI, RCTD, Tangram, where each box represents the method’s performance on the 50 paired datasets (five scRNA-seq datasets and ten single-cell spatial transcriptomics datasets). b UMAP plots of mouse retinal dataset (VZG105a_WT3) cells colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD using the mouse_LD_60 scRNA-seq dataset as the reference.

Techniques: Single-cell Transcriptomics, Comparison, Sampling, Sequencing

Journal: Genome Biology

Article Title: High-precision cell-type mapping and annotation of single-cell spatial transcriptomics with STAMapper

doi: 10.1186/s13059-025-03773-6

Figure Lengend Snippet: Application of STAMapper to MERFISH retina datasets. a Performance comparison of STAMapper and scANVI, RCTD, Tangram, where each box represents the method’s performance on the 50 paired datasets (five scRNA-seq datasets and ten single-cell spatial transcriptomics datasets). b UMAP plots of mouse retinal dataset (VZG105a_WT3) cells colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD using the mouse_LD_60 scRNA-seq dataset as the reference. AC amacrine cells, EC endothelial cells, MG Müller Glia, PC pericytes, RET reticulocyte, HC retinal horizontal cells, BC bipolar cells, Cones cone cells, RGC retinal ganglion cells, RPE retinal pigment epithelium, Rods Rod cells. c The heatmap of the marker expression for major cell types on the scRNA-seq dataset grouped by manual annotation and on the corresponding spatial transcriptomics dataset annotated by STAMapper, scANVI, and RCTD, respectively. d A schematic illustration of the distribution of cell types within the retina. e. Spatial organization of a slice from spatial transcriptomics dataset corresponding to ( b ), where cells are colored by the annotation by STAMapper, scANVI, and RCTD, respectively

Article Snippet: Fig. 3 Application of STAMapper to MERFISH retina datasets. a Performance comparison of STAMapper and scANVI, RCTD, Tangram, where each box represents the method’s performance on the 50 paired datasets (five scRNA-seq datasets and ten single-cell spatial transcriptomics datasets). b UMAP plots of mouse retinal dataset (VZG105a_WT3) cells colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD using the mouse_LD_60 scRNA-seq dataset as the reference.

Techniques: Comparison, Marker, Expressing

Journal: Genome Biology

Article Title: High-precision cell-type mapping and annotation of single-cell spatial transcriptomics with STAMapper

doi: 10.1186/s13059-025-03773-6

Figure Lengend Snippet: Application of STAMapper to MERFISH hypothalamic dataset. a UMAP plots of mouse hypothalamic dataset colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD, respectively. b Spatial organization of a slice from mouse hypothalamic dataset corresponding to ( a ), cells are colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD, respectively. c Sankey plot showing the accuracy of the cell-type annotation by STAMapper, scANVI, and RCTD, respectively. The left side of the Sankey plot represents manual annotations, while the right side shows the predicted results. The height of each linkage line reflects the number of cells. d Heatmap plot of marker expression for major cell types presented in manual annotation with mismatched labels between manual annotation and STAMapper. e Expression levels of Sema4d (a marker of OD Newly formed ) across different cell types (annotated by STAMapper). f The predicted probability of STAMapper for cells from spatial data (left panel) and unknown cells from spatial data (right panel), the red dash line indicates x = 0.738 in both panels. g Cell-type level distance from spatial data to single-cell data on cell embeddings learned by STAMapper. Bold indicates unknown cells were predicted as this specific cell type, and red denotes cell types present in single-cell data but not annotated in spatial data by manual annotation. h UMAP plots of the co-embedding of scRNA-seq and spatial data learned by STAMapper. Cells are colored by manual annotation, STAMapper prediction without unknown detection, and STAMapper prediction with unknown detection. The percentages in parentheses represent the predicted accuracy

Article Snippet: Fig. 3 Application of STAMapper to MERFISH retina datasets. a Performance comparison of STAMapper and scANVI, RCTD, Tangram, where each box represents the method’s performance on the 50 paired datasets (five scRNA-seq datasets and ten single-cell spatial transcriptomics datasets). b UMAP plots of mouse retinal dataset (VZG105a_WT3) cells colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD using the mouse_LD_60 scRNA-seq dataset as the reference.

Techniques: Marker, Expressing

Journal: Genome Biology

Article Title: High-precision cell-type mapping and annotation of single-cell spatial transcriptomics with STAMapper

doi: 10.1186/s13059-025-03773-6

Figure Lengend Snippet: Application of STAMapper to Nanostring HCC dataset. a UMAP plots of the human HCC dataset colored by STAMapper, scANVI, and RCTD, respectively. Macro macrophage, NK natural killer, DC dendritic cell. b Accuracy of STAMapper, scANVI, RCTD, and Tangram on human HCC dataset. c UMAP plot of the normalized marker expression corresponding to major cell types. d Spatial organization of ROI 1, cells are colored by the annotation of STAMapper, RCTD, and scANVI, respectively. e The normalized marker expression on ROI 1. f Density plot for the distribution of macro cells, annotated by STAMapper. g Physical distance of immune cells to malignant cells, with cells being annotated by STAMapper. h Boxplot for the scores of selected pathways (stemness, proliferation, and MHC-I) on malignant cells near macro and other malignant cells. i Heatmap displaying genes with the highest normalized attention weights, categorized by each cell type. We aggregate the normalized attention weights from that gene to all cells belonging to the cell type and compute their average as the cell type’s normalized attention weights. These scores reflect the gene’s overall contribution to the annotation of that cell type. j Cosine similarity of the gene embedding pairs learned by STAMapper, where gene pairs were TFs collected from hTFtarget. RELA - EPCAM and STAT3 - SMAD3 were validated to exist in HCC malignant cells in the literature

Article Snippet: Fig. 3 Application of STAMapper to MERFISH retina datasets. a Performance comparison of STAMapper and scANVI, RCTD, Tangram, where each box represents the method’s performance on the 50 paired datasets (five scRNA-seq datasets and ten single-cell spatial transcriptomics datasets). b UMAP plots of mouse retinal dataset (VZG105a_WT3) cells colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD using the mouse_LD_60 scRNA-seq dataset as the reference.

Techniques: Marker, Expressing

Journal: Genome Biology

Article Title: High-precision cell-type mapping and annotation of single-cell spatial transcriptomics with STAMapper

doi: 10.1186/s13059-025-03773-6

Figure Lengend Snippet: Application of STAMapper to Slide-tags human prefrontal cortex dataset. a , b UMAP plot for the co-embedding of scRNA-seq and spatial dataset learned by STAMapper. Cells are colored based on the prediction of STAMapper ( a ) and the origin of the datasets ( b ), respectively. Oligo oligodendrocytes, OPC oligodendrocyte progenitor cells. c The predicted cell-type probabilities for each cell (each column) in the spatial data. A maximum of 50 cells was subsampled from each type for visualization. d UMAP plots showing the co-embedding of the scRNA-seq and spatial dataset learned by STAMapper, cells are colored by the normalized expression levels of GPR17 . e Boxplots of the cosine similarity between gene embedding pairs grouped by the number of shared pathways. f UMAP plot for the distribution of gene embedding. Genes are colored by clusters identified through the Leiden algorithm. g Abstracted graph of the heterogenous cell-gene graph, where nodes represent cell types (pink) or gene modules (blue). Node size reflects the number of cells in a cell type or genes in a module. Edge width varies with the average expression levels of cell types linked to gene modules, determined by STAMapper. h UMAP plots showing the co-embedding of scRNA-seq and spatial dataset learned by STAMapper, cells are colored by the normalized expression levels of Module 12. i Enrichment analysis of gene module 12 related to Oligo_GPR17 cells. j Spatial organization of cells from the spatial dataset. Cells are clustered by STAGATE with resolution = 0.05. k The Normalized expression of SYT4 (marker gene of grey matter) and LRP2 (marker gene of white matter). l – n Spatial organization and UMAP plot of astrocyte ( l ), excitatory ( m ), and inhibitory ( n ) Subtypes predicted by STAMapper from the spatial dataset, Subtypes with more than 20 cells are shown. The UMAP coordinates are calculated from the expression of the spatial data

Article Snippet: Fig. 3 Application of STAMapper to MERFISH retina datasets. a Performance comparison of STAMapper and scANVI, RCTD, Tangram, where each box represents the method’s performance on the 50 paired datasets (five scRNA-seq datasets and ten single-cell spatial transcriptomics datasets). b UMAP plots of mouse retinal dataset (VZG105a_WT3) cells colored by the manual annotation and the prediction of STAMapper, scANVI, and RCTD using the mouse_LD_60 scRNA-seq dataset as the reference.

Techniques: Expressing, Marker